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| Registros recuperados : 5 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/10/2001 |
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Data da última atualização: |
28/12/2005 |
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Autoria: |
BESPALHOK FILHO, J. C.; KOBAYASHI, A. K.; PEREIRA, L. F. P.; HISSANO, Z.; VIEIRA, L. G. E. |
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Afiliação: |
UEL; IAPAR. |
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Título: |
In vitro adventitious shoot regeneration from sweet orange using thin epicotyl sections. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v.1, n.1, p.27-34, 2001. |
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ISSN: |
1518-7853 |
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Idioma: |
Inglês |
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Conteúdo: |
A system for in vitro plant regeneration from thin transversal epicotyl sections of sweet orange (Citrus sinensis L. Osbeck cvs. Pera, Valencia and Folha Murcha) was developed. Thin section explants (1-2 mm) from seedlings produced adventitious shoot buds on MS medium supplemented with BA (0.5-5 µM). Optimal shoot induction was achieved on 2-5 µM BA. Explants with buds were transferred to elongation media in order to improve the recovery of normal shoots. Higher number of elongated shoots was obtained on medium with 0.5 µM BA. Elongated shoots were rooted on half strength MS medium without growth regulators and transferred to soil for acclimatization. The advantages of this system for transformation are discussed. |
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Palavras-Chave: |
Adventitious buds; Epicotyl; Orange; Regeneration; Shoot multiplication. |
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Thesagro: |
Citrus Sinensis; Laranja; Regeneração. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01510naa a2200277 a 4500
001 1653506
005 2005-12-28
008 2001 bl uuuu u00u1 u #d
022 $a1518-7853
100 1 $aBESPALHOK FILHO, J. C.
245 $aIn vitro adventitious shoot regeneration from sweet orange using thin epicotyl sections.
260 $c2001
520 $aA system for in vitro plant regeneration from thin transversal epicotyl sections of sweet orange (Citrus sinensis L. Osbeck cvs. Pera, Valencia and Folha Murcha) was developed. Thin section explants (1-2 mm) from seedlings produced adventitious shoot buds on MS medium supplemented with BA (0.5-5 µM). Optimal shoot induction was achieved on 2-5 µM BA. Explants with buds were transferred to elongation media in order to improve the recovery of normal shoots. Higher number of elongated shoots was obtained on medium with 0.5 µM BA. Elongated shoots were rooted on half strength MS medium without growth regulators and transferred to soil for acclimatization. The advantages of this system for transformation are discussed.
650 $aCitrus Sinensis
650 $aLaranja
650 $aRegeneração
653 $aAdventitious buds
653 $aEpicotyl
653 $aOrange
653 $aRegeneration
653 $aShoot multiplication
700 1 $aKOBAYASHI, A. K.
700 1 $aPEREIRA, L. F. P.
700 1 $aHISSANO, Z.
700 1 $aVIEIRA, L. G. E.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv.1, n.1, p.27-34, 2001.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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